western blotting detection kit Search Results


vectastain abc reagent  (Vector Laboratories)
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Vector Laboratories vectastain abc reagent
Vectastain Abc Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemiluminescence ecl western blotting detection kit  (GE Healthcare)
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GE Healthcare chemiluminescence ecl western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Chemiluminescence Ecl Western Blotting Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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westernbright sirius western blotting detection kit  (Advansta)
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Advansta westernbright sirius western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Westernbright Sirius Western Blotting Detection Kit, supplied by Advansta, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc kit diaminobenzadine (dab  (Vector Laboratories)
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Vector Laboratories vectastain abc kit diaminobenzadine (dab
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Vectastain Abc Kit Diaminobenzadine (Dab, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blotting detection kit  (Advansta)
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Advansta western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Western Blotting Detection Kit, supplied by Advansta, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blot kit  (Beijing Solarbio Science)
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Beijing Solarbio Science western blot kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Western Blot Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl kit 4aw011-100  (4A Biotech)
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4A Biotech ecl kit 4aw011-100
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Kit 4aw011 100, supplied by 4A Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl western blotting detection kit  (TransGen biotech co)
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TransGen biotech co ecl western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Detection Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl western blotting substrate 07880  (Nacalai)
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Nacalai ecl western blotting substrate 07880
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Substrate 07880, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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visualization reagent absignal abc-3001  (AbClon Inc)
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AbClon Inc visualization reagent absignal abc-3001
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Visualization Reagent Absignal Abc 3001, supplied by AbClon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prometheus prosignal pico western blotting detection kit  (Genesee Scientific)
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Genesee Scientific prometheus prosignal pico western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Prometheus Prosignal Pico Western Blotting Detection Kit, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kwikquant western blot detection kit  (Kindle Biosciences)
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Kindle Biosciences kwikquant western blot detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Kwikquant Western Blot Detection Kit, supplied by Kindle Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with a monoclonal mouse antibody, clone 303.9 (ARP), and ECL (Amersham Pharmacia).

Journal:

Article Title: Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5- rep Sequence in cis

doi: 10.1128/JVI.76.11.5411-5421.2002

Figure Lengend Snippet: Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with a monoclonal mouse antibody, clone 303.9 (ARP), and ECL (Amersham Pharmacia).

Article Snippet: AAV Rep 78 protein was detected with a monoclonal mouse antibody supernatant, clone 303.9 (ARP), and an enhanced chemiluminescence (ECL) Western blotting detection kit (Amersham Pharmacia), according to the manufacturer's instructions.

Techniques: Expressing, Infection, Purification